Dr. Shubham Shrivastava
Profile
Date of Birth: 15- 2-1982
Qualification: PhD
Designation: Research Scientist
Contacts
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: Phone: +65-94606909
ACADEMIC QUALIFICATIONS:
| Degree | Year | Institution and Location | Major Subjects |
| Research Scientist | Present | Singapore - MIT Alliance for Research & Technology (SMART), Singapore | Natural killer cells in Dengue Virus Pathogenesis |
| Postdoctoral Fellow | February, 2015 | Saint Louis University, Saint Louis, Missouri, USA | Hepatitis C Virus mediated innate immunity |
| Ph.D | April, 2010 | National Institute of Virology, Pune, Maharashtra, India | Vaccine development against hepatitis B and hepatitis E viruses |
| Master of Science | 2004 | Kumaun University, Nainital, Uttarakhand, India | Biotechnology |
| Bachelor of Science | 2002 | Ranchi University, Ranchi, Jharkhand, India | Botany (Hons.), Chemistry, Zoology |
Research experience:
“Mechanism of Natural Killer Cell Activation in controlling Dengue virus infection in Humanized mouse model”
In humanized mouse model of Dengue infection, human NK cell control viremia in Interferon-γ dependent manner. NK cell activation is dependent on cell to cell contact with virus infected dendritic cells.
Postdoctoral Fellow, Department of Pathology, Saint Louis University, Saint Louis, USA “Hepatitis C Virus mediated innate immunity”
- Hepatitis C virus induced autophagy and its role in viral pathogenesis
HCV infection impairs innate immune response by inducing autophagy in hepatocytes. Autophagy induction also help virus to facilitate infectious virus particle production and establish infection in hepatocytes - Molecular mechanism involved in evasion of innate immune response by Hepatitis C virus
HCV infection suppresses interferon alpha induced interferon stimulated gene expression proteins, such as, ISG56 and IFITM1 in hepatocytes. HCV directly reduces interferon alpha production by inhibiting nuclear translocation of IRF7. - HCV infection and modulation of microRNA in virus mediated pathogenesis
MicroRNA profiling in HCV infected patients reveals gradual increase in miR-20a expression with increase in severity of liver fibrosis. HCV infection suppresses expression of miR-181c to enhance HOXA1 expression and cell growth in hepatocytes.
Ph.D, Hepatitis Department, National Institute of Virology, Pune, Maharashtra, India “Development of Combined DNA Vaccine for Hepatitis B and E Viruses”
- Characterization of humoral and cellular response in candidate combination vaccine utilizing liposome encapsulation approaches of DNA, protein and DNA + protein formulations against hepatitis B and hepatitis E in mice
- Anti-HBs and anti-HEV IgG titres of individual component of DNA+ protein formulations were comparable to respective titres in combination vaccine of DNA+ protein formulations
- Equivalent levels of IgG1 and IgG2a were observed in combination vaccine group against both components of the vaccine.
- Combination vaccine group showed mixed Th1/Th2 cytokine profile.
Honors and Awards:
| 2002-2004 | M.Sc Scholarship from Department of Biotechnology (DBT) |
| 2004 | Qualified GATE (Graduate Aptitude Test in Engineering) |
| 2004 | Qualified CSIR/University Grant Commission (UGC) - CSIR Junior Research Fellowship (June, 2004) |
| 2005-2009 | Ph.D Fellowship from ICMR (Indian Council of Medical Research) |
| 2007 | Best Oral Presentation in 32nd Indian Immunological Society in Pune, India |
| 2011 | Travel Award, International Symposium on Hepatitis C virus and related viruses |
| 2012 | Travel Award, American Society of Virology |
Membership:
- American Society of Virology, 2012
Publications
- Arankalle VA, Shrivastava S, Cherian S, Gunjikar RS, Walimbe AM, Jadhav SM, Sudeep AB, Mishra AC. Genetic divergence of Chikungunya viruses in India (1963- 2006) with special reference to the 2005-2006 explosive epidemic. J Gen Virol 2007; 88:1967-76. Impact factor: 3.36
- Arankalle VA, Lole KS, Deshmukh TM, Srivastava S, Shaligram US. Challenge studies in Rhesus monkeys immunized with candidate hepatitis E vaccines: DNA, DNA-prime- protein-boost and DNA-protein encapsulated in liposomes. Vaccine 2009; 27:1032-9. (Ph.D work) Impact factor: 3.77
- Shrivastava S, Lole KS, Tripathy AS, Shaligram US, Arankalle VA. Development of candidate combination vaccine for hepatitis E and hepatitis B: a liposome encapsulation approach. Vaccine 2009; 27:6582-8. (Ph.D work) Impact factor: 3.77
- Raychoudhuri A, Shrivastava S, Steele R, Dash S, Kanda T, Ray R, Ray RB. Hepatitis C virus infection impairs IRF-7 translocation and interferon-alpha synthesis in immortalized human hepatocytes. J Virol 2010; 84:10991-8. Impact factor: 5.08
- Shrivastava S, Raychoudhuri A, Steele R, Ray R, Ray RB. Knockdown of autophagy enhances the innate immune response in hepatitis C virus-infected hepatocytes. Hepatology 2011; 53:406-414. Impact factor: 12.00
- Raychoudhuri A, Shrivastava S#, Steele R, Kim H, Ray R, Ray RB. ISG56 and IFITM1 proteins inhibit hepatitis C virus replication. J Virol 2011; 85:12881-9. (#shared first author) Impact factor: 5.08
- Bose SK, Shrivastava S, Meyer K, Ray RB, Ray R. Hepatitis C virus activates the mTOR/S6K1 signaling pathway in inhibiting IRS-1 function for insulin resistance. J Virol 2012; 86:6315-22. Impact factor: 5.08
- Shrivastava S, Bhanja Chowdhury J, Steele R, Ray R, Ray RB. Hepatitis C Virus Upregulates Beclin1 for Induction of Autophagy and Activates mTOR Signaling. J Virol 2012; 86:8705-12. Impact factor: 5.08
- Bhanja Chowdhury J, Shrivastava S, Steele R, Di Bisceglie AM, Ray R, Ray RB. Hepatitis C Virus infection modulates interferon stimulatory gene IFITM1 by up- regulating miR-130a. J Virol 2012; 86:10221-5. Impact factor: 5.08
- Shrivastava S, Petrone J, Steele R, Lauer GM, Di Bisceglie AM, Ray RB. Upregulation of circulating miR-20a is correlated with Hepatitis C Virus mediated liver disease progression. Hepatology. 2013; 58:863-71 (selected as editorial of Hepatology journal in September 2013) Impact factor: 12.00
- Bittar C, Shrivastava S, Bhanja Chowdhury J, Rahal P, Ray R. Hepatitis C Virus NS2 protein inhibits DNA damage pathway by sequestering p53 to the cytoplasm. PLoS One 2013; 8:e62581. Impact factor: 4.09
- . Shrivastava S, Mukherjee A, Ray R, Ray RB. Hepatitis C Virus Induces Interleukin-1β (IL-1β)/IL-18 in Circulatory and Resident Liver Macrophages. J Virol 2013; 87:12284-90. Impact factor: 5.08
- Shrivastava S, Mukherjee A, Ray RB. Hepatitis C virus infection, microRNA and liver disease progression. World J Hepatol 2013; 5:479-486. Review
- Shrivastava S, Ray RB. Hepatitis C virus infection, autophagy and innate immune response. In: Hayat MA, ed. Autophagy. Cancer, Other Pathologies, Inflammation, Immunity, Infection and Aging. Academic Press, Elsevier. 2014; 3:164-190.
- . Mukherjee A, Shrivastava S#, Bhanja Chowdhury J, Ray R, Ray RB. Transcriptional Suppression of miR-181c by Hepatitis C Virus Enhances Homeobox A1 Expression. J Virol 2014; 88:7929-40. (# shared first author) (selected as “Spotlight” in July issue of Journal of Virology) Impact factor: 5.08
- Shrivastava S, Steele R, Ray R, Ray RB. MicroRNAs: Role in Hepatitis C Virus pathogenesis. Genes Dis. 2015; 2:35-45. Review
- Shrivastava S, Devhare P, Sujijantarat N, Steele R, Kwon YC, Ray R, Ray RB. Knockdown of autophagy inhibits infectious hepatitis C virus release by exosomal pathway. J Virol 2015 Nov 18. pii: JVI.02383-15. (Ahead of print)
WORKSHOPS and CONFERENCES:
| October, 2007 | Training course on “TaqMan Low Density Array” organized by Lab India Research and Development Laboratory, Gurgoan, India |
| November, 2008 | Training Programme on “Flow Cytometry, handling and operation of FACS Calibur” organized by BD Biosciences, Gurgaon, Delhi, India |
| December, 2007 | Oral Presentation on “Study of Immune Response to a Combination Vaccine against Hepatitis B and Hepatitis E in mice” in 32nd Indian Immunological Society in Pune, India |
| December, 2007 | Poster presentation on “Enhancement of humoral immune response to a combination vaccine against Hepatitis B surface antigen, HBsAg and Hepatitis E partial capsid protein, NE” in Indian Conference of Virological Society (ICVT) Delhi, India |
| October, 2009 | Poster presentation on “Development of Combination Vaccine against Hepatitis B and Hepatitis E viruses: a liposome encapsulation approach” in 3rd Vaccine Congress, Singapore |
| September, 2011 | Oral presentation on “Hepatitis C virus infection induces autophagy and blocks innate immune response” at 18th International Symposium on Hepatitis C virus and related viruses, Seattle, Washington, USA |
| July, 2012 | Oral presentation on “Hepatitis C virus mediated autophagy activates mTOR signaling pathway” at 31st Annual Meeting, American Society of Virology, Madison, Wisconsin, USA |
| January, 2014 | Poster presentation on “Hepatitis C Virus induces secretion of Interleukin- 1β and IL-18 by human macrophages” at Keystone Symposia Conference on “Innate Immunity to Viral Infections”, Keystone, Colorado, USA |
References
- Graduate Advisor: Dr. V A Arankalle, Scientist “G” & Head, Hepatitis Division, Microbial Containment Complex, National Institute of Virology, 130/1 Sus Road, Pashan, Pune - 411021, Maharashtra, India. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
- Postdoctoral Advisor: Dr. Ratna B. Ray, Professor, Department of Pathology, Edward A Doisy Research Center, RM 207, Saint Louis University, 1100 South Grand Blvd., Saint Louis, MO, 63104, USA. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
- Dr. Ranjit Ray, Professor, Department of Internal Medicine, Edward A Doisy Research Center, RM 823, Saint Louis University, 1100 South Grand Blvd., Saint Louis, MO, 63104, USA. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.