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Interactive Research School For Health Affairs (IRSHA), Pune

Facilities

Biochemistry

Gas Chromatograph Systems, Refrigerated table top Centrifuge, -80 Deep Freezer, -20 Deep Freezer, UV/VIS Spectrophotometer, Balances, pH meter, Clinical Centrifuges, Ultra sonic cleaner, HPLC, Cooling Centrifuge, Ice Flaking Machine

Cell/Tissue Culture

CO2 Incubators, Laminar Flow Cabinets, Eliza plate Reader, Spectro Flurometer, BOD Incubator, Cooling Incubator, -80 Deep Freezer, -20 Deep Freezer, Incubator Shaker, Axiostar Microscopes, Water PRO RO, PRO PS Integra Micromanipulator system, Inverted Microscope Flurescence with camera, Cryo Cans

Molecular Biology

RT-PCR, PCR, Centrifuge, Incubator Shaker, Speed Vac System, Gel Documentation System, Hybridization Oven, Sorwall Highspeed refrigerated Centrifuge, Nano drop Microbial & Fermentation facility: Laminar Flow Cabinets, Fermentor

Washing facility:

Glassware and Plasticware washing, packing, autoclaving and sterilizing facility

Gas Chromatography Cooling Centrifuge
Gas chromatography is used to estimate the various fatty acids in biological and food samples. These equipments are used for centrifugation of samples at low temperature. Extraction of DNA, RNA, Proteins etc. frequently require the use of cooling centrifuges.
HPTLC Spectrophotometer

A Camag high-pressure thin-layer chromatography system equipped with an automatic TLC sampler (Linomat 5), TLC scanner 3, and integrated software Win-Cats version 4.

Note on application of HPTLC

  • Qualitative and quantitative analysis of active compounds in medicinal plant/herbal/synthetic drugs
  • HPTLC fingerprint of extracts/fractions/formulations.
  • Preparative HPTLC for isolation of active constituent from extracts/herbal drugs.
  • Toxic/medicinally active food components present in food.
  • Pharmaceutical analysis of bulk drugs.
  • Pharmacokinetic studies.
Principle- Ultraviolet–visible spectroscopy or ultraviolet-visible spectrophotometry (UV Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved.

Make/Model- Perkin Elmer, Lambda 25

Specification/Features - Solid, Liquid, Paste and powder samples - Range: 190 nm - 1100 nm; Bandwidth: 0.5 nm - 4 nm (variable)

Applications- Qualitative and quantitative analysis of different analytes such as biological macromolecules, transition metal ions and highly conjugated organic compounds, determination of the functional groups within a molecule, etc

Biosafety cabinets level II Fluorescence plate Reader

Biosafety cabinets level II

BMG Labtech fluorescence microplate reader
ELISA plate reader Biosafety cabinets level I

ELISA plate reader

Biosafety cabinets level I
CO2 Incubators Inverted microscope

CO2 Incubators

Inverted microscope
PCR System Gel Documentation

The Real time PCR system is used to monitor the amplification of targeted DNA molecules during PCR. It is used to study the expression of candidate genes in cells or tissues.

High-performance liquid chromatography (HPLC) is used to estimate the levels of vitamins, one-carbon metabolites and various amino acids.
Incubator Shaker Nano Drop

The equipment is essential to maintain the temperature and aeration (through shaking) for microbial culture growth. Bacterial culture and suspension cell cultures can be incubated in these equipments to provide optimum temperature and aeration for growth of the cultures.

The bio-photometer is used to estimate the purity and quantity of nucleic acids and protein quantity in very small volumes of 1-3ul. It can also be used for estimating bacterial growth and colorimetric protein assays.
Laminar Air flow Thermal cycler

Laminar air flow hood

Gradient Thermal cycler
Soxhlet Apparatus Digital Plethysmometer

Principle- A Soxhlet extractor is a piece of laboratory apparatus used when desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent. Normally a solid material containing some of the desired compound is placed inside a thimble made from thick filter paper, which is loaded into the main chamber of the Soxhlet extractor. After extraction the solvent is removed, typically by means of a rotary evaporator, yielding the extracted compound. The non-soluble portion of the extracted solid remains in the thimble, and is usually discarded.

Applications- Preparation of plant extracts, etc

Principle- The Digital Plethysmometer is a micro controlled volume meter, specially designed for accurate measurement of the rat / mouse paw swelling. The water displacement produced by the immersion of the animal paw in the measuring tube is reflected in to the second tube, inducing the volume displacement. This displacement value is recorded.

Make/Model-Orchid Scientific, PLM Digital Plethysmometer

Applications- Evaluation of the inflammatory response experimentally induced in small rodents and to screen potential anti-inflammatory or anti-edema properties of pharmacological substance

Spectrophotometer BioTek Epoch

Principle- Ultraviolet–visible spectroscopy or ultraviolet-visible spectrophotometry (UV Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved.

Make/Model- Perkin Elmer, Lambda 25

Specification/Features - Solid, Liquid, Paste and powder samples - Range: 190 nm - 1100 nm; Bandwidth: 0.5 nm - 4 nm (variable)

Applications- Qualitative and quantitative analysis of different analytes such as biological macromolecules, transition metal ions and highly conjugated organic compounds, determination of the functional groups within a molecule, etc

BioTek Epoch Micro-plate reader

Principle-BioTek Epoch Micro-plate reader is an instrument used for specific colorimetric assay done in 96-well microtitre plates. The instrument contains a set of optical filters that allow selective transmission of light of specific wave length through the samples and measures the relative transmittance with respect to the incident intensity. The fraction of light transmitted is related to the concentration of the molecules of interest in the medium. This system works in 96-well plate format generating data with high throughput. The instrument contains a separate data acquisition system and supports multiple data collection at different wavelengths.

Make / Model- BioTek Epoch Micro-plate reader

Specification/features- Monochromator-base UV-Vis wavelength selection, 200 nm to 999 nm wavelength range in 1 nm increments

Applications- ELISA, protein assays (Bradford, Lowry and Bicinchoninic acid assay), MTT assay, turbidity based bacterial growth kinetics study, wavelength scanning, etc.

Rotary Evaporator  
 

Principle- A rotary evaporator is a device used in chemical laboratories for the efficient and gentle removal of solvents from samples by evaporation. It involves evaporation of solvent (single-stage or straight distillation) under vacuum. The evaporator consists of a heating bath with a rotating flask, in which the liquid is distributed as a thin film over the hot wall surfaces and can evaporate easily. The evaporation rate is regulated by the heating bath temperature, the size of flask, the pressure of distillation and the speed of rotation.

Make/Model-IKA RV 10 Basic

Applications- Concentration of plant extracts, etc.

 
Symposium

2nd David Barker Memorial Symposium on Developmental Origins of Health and Disease (DOHaD) on Saturday 18th February 2017

CME by Ministry of AYUSH

CME on Basic Research Techniques for Ayurvedic PG Teachers Contact: Dr. Supriya Bhalerao - 27th February to 4th March 2017

World Obesity Day

On occasion of World Obesity Day, a workshop on Anthropometric assessment was organized by Obesity Research Lab, IRSHA on 13th October 2016. Dr. Sanjay Juvekar and Dr. Hemangi Lubree from Vadu Rural Health Program (VRHP), an initiative of KEM Hospital delivered a lecture on basic techniques of anthropometry followed by demonstration of the anthropometry assessment on volunteers by their team.

National Ayurveda Day

Ministry of AYUSH has declared Dhanateras to be celebrated as National Ayurveda Day. Prof. M S Baghel (Ex- Chairman, Ayurveda Chair University of Debrecan, Hungary) was the special invitee for the auspicious day. He threw light over the Ayurveda concepts of Diabetes, its consequences and its management. A lecture was delivered by Dr. Jyoti Shirodkar (Ayurvedic Diabetologist, Asso. Prof. Dept of Dravyaguna, TAMV) based on the theme Prevention and Control of diabetes using Ayurveda.

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