Dr.Ruchika Kaul-Ghanekar

Profile

Date of Birth: 27-04-1972

Qualification: MSc (Microbiology), PhD (Biotechnology)

Designation: Associate Professor, and Head Cancer Research Lab

Contacts

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Phone: 020-24366920 (ext.202)

Research experience:

  • Associate Professor, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Pune, India (2007 – till date);
  • Scientist “C” in Nanobiotechnolgy Division at Agharkar Research Institute, (Department of Science and Technology (DST), Govt. of India Institute (2006-07)
  • Research Associate at Nanobiotechnolgy Division at Agharkar Research Institute, (Department of Science and Technology (DST), Govt. of India Institute (2005-06)
  • DBT-Post Doctoral Research Fellow at Department of Biotechnology, Pune University, India (2004-05)
  • Teaching/Research Associate at Department of Biotechnology, Pune University, India (2003-04)

Area of interest:

  • Cancer Biology, Cell and Molecular Biology, Protein Biochemistry

Honors/awards:

  • Bharati Vidyapeeth’s “Best Researcher Award”, April 2011 for research excellence
  • Scientific Advisory Committee (SAC) member of CCRUM (Center Council of Research for Unani Medicine), Ministry of AYUSH, Govt of India.
  • Chairman for Nanobiology subject of MTech Nanotechnology, College of Engineering, Bharati Vidyapeeth University, Pune

Other activities

  • Reviewer for many peer reviewed journals

Professional memberships:

  • Member, Society for Ethnopharmacology- India, Pune Chapter
  • Life Member of Indian Society for Developmental Biologists (ISDB)
  • Life member of Society of Indian Cell Biology
  • Life Member of Mumbai Immunology Group

Publications:54

  • Articles: 44
  • General Articles: 5
  • Book Chapters: 4
  • Proceedings: 1

Research Programmes

Breast and Cervical Cancers account for the most common malignancy among women worldwide and are the leading cause of cancer deaths. In India, breast cancer is the first leading cause of mortality followed by cervical cancer. Despite advanced therapeutics, the serious adverse events (AEs) associated with the conventional treatments and the problem of recurrence remains inevitable. Accumulated evidences suggest that nutritive and non-nutritive plant-based agents can not only inhibit the process of carcinogenesis but can also prevent the AEs providing a very effective and affordable therapy for mass population.

My research group focuses on evaluation of natural products (nutraceuticals, medicinal plants, Ayurvedic and Homeopathic medicines) for their anticancer activity using cell and animal experimental models. We have established mechanistic approach in the effective use of nutritional and natural products to get translated into novel interventions/therapeutics against these cancers. In cervical cancer, we have established the anticancer potential of Cinnamon, Ficus religiosa and Alpha linolenic acid (an omega 3 fatty acid). In breast camncer, we have established the anticancer potential of a polyherbal formulation, homeopathic formulation of Terminalia chebula as well as EPA and DHA omega 3 fatty acids. Low ratios of n6/n3 (1:2.5, 1:4, 1:5, 1:10) fatty acids decreased the viability and growth of breast cancer cells and modulated lipid peroxidation, total cellular fatty acid composition and expression of tumor regulatory Matrix Attachment Region binding proteins (MARBPs). Fish oil (EPA and DHA) supplementation in breast cancer patients undergoing chemotherapy significantly increased their antioxidant status and improved their quality of life. In breast cancer cells, we established the hyperthermia and anticancer potential of a nanoconjugate comprising of cinnamaldehyde, bioactive component of Cinnamon that was tagged to magnetic nanoparticles.

Publications

  • Kirtee Wani, Nilesh Shah, Asmita Prabhune, Arun Jadhav, Prabhakar Ranjekar and Ruchika Kaul-Ghanekar. Evaluating the anticancer activity and nanoparticulate nature of homeopathic preparations of Terminalia chebula (TC). 2016. Homeopathy (accepted).
  • Parul Dubey, Prerna Raina, Asmita Prabhune and Ruchika Kaul-Ghanekar. Cetyl alcohol and oleic acid sophorolipids exhibit anticancer activity. (Accepted in International Journal of Pharmacy and Pharmaceutical Sciences, 2016).
  • Prerna Raina, Deepak M, Chandrasekaran CV, Amit Agarwal, Narendra Wagh and Ruchika Kaul-Ghanekar. Comparative analysis of anti-inflammatory activity of aqueous and methanolic extracts of Ocimum basilicum in RAW264.7, SW1353 and human primary chondrocytes. Accepted in Journal of Herbal Medicine, 2016
  • P Raina, CV Chandrasekaran, M Deepak , A Aggarwal and K-G Ruchika. Evaluation of subacute toxicity of methanolic/aqueous preparation of aerial parts of O.sanctum in Wistar rats: clinical, hematological, biochemical and histopathological studies. J Ethnopharmacol. 2015 Dec; 175:509-17.
  • Rashmi Deshpande, Prakash Mansara and Ruchika Kaul-Ghanekar. Alpha linolenic acid regulates Cox2/VEGF/MAP kinase pathway and decreases the expression of HPV oncoproteins E6/E7 through restoration of p53 and Rb expressionin human cervical cancer cell lines Tumour Biol. 2015 Oct 6
  • Prerna Raina, CV Chandrasekaran, Amit Aggarwal, Narendra Wagh and Ruchika Kaul-Ghanekar. Comparative analysis of anti-inflammatory activity of aqueous and methanolic extracts of C. cassia and C. zeylanicum in RAW264.7, SW1353 and primary chondrocytes. International Journal of Pharmacy and Pharmaceutical Sciences, 7: 392-396, 2015.
  • Snehal A Suryavanshi, Kavita Shinde-Kadam, Prerna Raina, Ravindra Nimbargi, Vijaya A Pandit and Ruchika Kaul-Ghanekar. Evaluation of acute and sub-acute toxicity of a standardized polyherbal formulation (HC9): an in vivo study. International Journal of Pharmacy and Pharmaceutical Sciences, 7:110-117, 2015.
  • Prakash P. Mansara, Rashmi A. Deshpande, Milind M. Vaidya, Ruchika Kaul-Ghanekar. Differential ratios of omega fatty acids (AA/EPA+DHA) modulate growth, lipid peroxidation and expression of tumor regulatory MARBPs in breast cancer cell lines MCF7 and MDA-MB-231. PlosOne, 10(9):e0136542, 2015.
  • Kirtee Wani, Kirtan Tarawadi and Ruchika Kaul-Ghanekar. Nanocarriers for delivery of herbal based drugs in breast cancer-an overview. Journal of Nano Research 34: 29-40, 2015.
  • Prakash Mansara, Mrunal Ketkar, Rashmi Deshpande, Amol Chaudhary, Kavita Shinde, Ruchika Kaul-Ghanekar. Omega-3 fatty acid supplementation in breast cancer patients undergoing chemotherapy improved their antioxidant status - a case series. J Med Case Reports, 9:148, 2015.
  • Pradeep Kumar Singha, Kirtee Wani, Ruchika Kaul-Ghanekar, Asmita Prabhune and Satishchandra Ogale. From Micron to Nano-Curcumin by sophorolipid co-processing: Highly enhanced bioavailability, fluorescence, and anti-cancer efficacy. RSC Advances, 4: 60334-41, 2014.
  • Kirtee D. Wani, Brijesh S. Kadu, Preeti Gupta, Avinash V. Deore, Rajeev C. Chikate, Pankaj Poddar, Sanjay D. Dhole and Ruchika Kaul-Ghanekar*. Synthesis, characterization and in vitro study of biocompatible Cinnamaldehyde functionalized magnetite nanoparticles (CPGF NPs) for hyperthermia and drug delivery applications in breast cancer. PlosOne, 9: e107315; 2014
  • Snehal Suryavanshi, Anand Zanwar, Mahabaleshwar Hegde and Ruchika Kaul-Ghanekar*. Standardization of a polyherbal formulation (HC9) and comparative analysis of its cytotoxic activity with respect to the individual herbs present in the composition in breast cancer cell lines. Pharmacognosy Journal 6: 87-95, 2014.
  • Amit S Choudhari, Prerna Raina, Manasi M Deshpande, Ashok G Wali, Anand Zanwar, Subhash L Bodhankar, Ruchika Kaul-Ghanekar*. Evaluating the anti-inflammatory potential of Tectaria cicutaria L. rhizome extract in vitro as well as in vivo. Journal of Ethnopharmacology 150 215–222, 2013.
  • Amit S. Choudhari, Snehal A. Suryavanshi and Ruchika Kaul-Ghanekar*. The aqueous extract of Ficus religiosa induces cycle arrest in human cervical cancer cell lines SiHa (HPV-16 positive) and apoptosis in HeLa (HPV-18 positive). 2013. PlosOne [The abstract of paper was featured in World Biomedical Frontiers (http://biomedfrontiers.org/cancer-2014-5-7/ ].
  • Synthesis and characterization of gold nanoparticles using Ficus religiosa extract. Kirtee Wani, Amit Choudhari, Rajeev Chikate and Ruchika Kaul-Ghanekar*. 2013. Carbon – Sci. Tech. 5/1 (2013) 203 - 210
  • Rashmi Deshpande, Prakash Mansara, Snehal Suryavanshi, Ruchika Kaul-Ghanekar*. Alpha-linolenic acid regulates the growth of breast and cervical cancer cell lines through regulation of NO release and induction of lipid peroxidation. 2013. Journal of Molecular Biochemistry.
  • Ruchika Kaul-Ghanekar*, Prerna Raina. Potential of Nutraceuticals and Medicinal Plants in the Management of Osteoarthritis. Review. Acta Biologica Indica 2012,1(1):27-46.
  • Mithun Bandivadekar, Shyamsundar Pancholi, Ruchika Kaul-Ghanekar, Amit Choudhari, and Soumya Koppikar. Single non-ionic surfactant based self-nanoemulsifying drug delivery systems: formulation, characterization, cytotoxicity and permeability enhancement study. Drug Development and Industrial Pharmacy, 2012; Early Online: 1–8
  • Kale S, Jadhav A, Verma S, Koppikar S, Kaul-Ghanekar R, Dhole S, Ogale S. 2011. Characterization of biocompatible NiCo2O4 nanoparticles for applications in hyperthermia and drug delivery. Nanomedicine 2012 May;8(4):452-9. Epub 2011 Aug 10.
  • Amit Subhash Choudhari, Snehal Suryavanshi, Harshad Ingle, Ruchika Kaul-Ghanekar*. Evaluating the antioxidant potential of aqueous and alcoholic extracts of Ficus religiosa using ORAC assay and assessing their cytotoxic activity in cervical cancer cell lines. Biotechnol. Bioinf. Bioeng. 2011, 1(4):443-450
  • Snehal Suryavanshi, Amit Choudhari, Rucha Deshpande, Omkar Kulkarni, Ruchika Kaul-Ghanekar*. Analyzing the antioxidant potential of aqueous and ethanolic preparations of a herbal composition (HC9) and evaluating their cytotoxic activity in breast cancer cell lines. Biotechnol. Bioinf. Bioeng. 2011, 1(4):513-522
  • Wani K, Ahmed A, Kitture R, Koppikar S, Choudhari A, Kale S, Kaul-Ghanekar R*. Synthesis, characterization and in vitro study of curcumin-functionalized citric acid-capped magnetic (CCF) nanoparticles as drug delivery agents in cancer. Journal of Bionanoscience Vol. 5, 59–65, 2011.
  • Bandivadeka MM, Pancholi SS, Kaul-Ghanekar R, Choudhari A, Koppikar S. Self-microemulsifying smaller molecular volume oil (Capmul MCM) using non-ionic surfactants: a delivery system for poorly water-soluble drug. Drug Dev Ind Pharm. 2011 Nov 16.
  • Malonia SK, Sinha S, Laksminarsimhan P, Singh K, Jalota Badhwara, Rampalli S, Kaul-Ghanekar R & Chattopadhay S. Gene regulation by SMARI: Role in cellular homeostasis & cancer, Biochim Biophys Acta. 2011 Jan;1815(1):1-12. Epub 2010 Aug 13. Review.
  • Kittur A, Koppikar S, Kaul-Ghanekar R & Kale S. N. Catalyst efficiency, photostability & reusability study of ZnO nanoparticles in visible height for dye degradation. Journal of Physics & Chemistry of Solids, 2011, 72(1): 60-66.
  • Koppikar S, Choudhari A, Suryawanshi S, Kumari S, Chattopadhay S and Kaul-Ghanekar R*. Aqueous Cinnamon extract (ACE-c) from bark of Cinnamomum cassia causes apoptosis through loss of mitochondrial membrane potential. BMC Cancer 2010; 10: 210.
  • Singh R, Koppikar S, Paul P, Gilda S, Paradkar AR and Kaul-Ghanekar R*. Comparative Analysis of Cytotoxic Effect of Aqueous Cinnamon Extract (ACE) from Cinnamomum zeylanicum Bark with Commercial Cinnamaldehyde on Various Cell lines. Pharmaceutical Biology (2009); 47: 1174-1179.
  • Kaul-Ghanekar R, Singh S, Mamgain H, Jalota-Badhwar A, Paknikar KM and Chattopadhyay S. Tumor suppressor protein SMAR1 modulates the roughness of cell surface: combined AFM and SEM study. BMC Cancer 2009, 9:350
  • Pandey, V., M. V. Vijayakumar, Kaul-Ghanekar R, Mamgain H, Paknikar KM and Bhat MK. Atomic Force Microscopy, biochemical analysis of 3T3-L1 cells differentiated in the absence and presence of insulin. Biochim Biophys Acta. 2009,1790:57-64.
  • Kale S N, Kitture R, Koppikar S J, Kaul-Ghanekar R, Patil S I. Role Of Zno Bulk And Nanopowders in Photocatalytic Decolorisation of Textile Industrial Dyes. The Abdus Salam International Centre For Theoretical Physics, 2009
  • Dipak Maity, Sangeeta Kale, Ruchika Kaul-Ghanekar, Jun-Min Xue, Jun Ding. Studies of magnetite nanoparticles synthesized by thermal decomposition of iron (III) acetylacetonate in tri(ethylene glycol). Journal of Magnetism and Magnetic Materials (2009); 321: 3093-3098.
  • Sandip Dhobale, Trupti Thite, Soumya J. Koppikar, S. L. Laware, Ruchika-Kaul Ghanekar and S. N. Kale. Zinc Oxide Nanorods as alpha-amylase Inhibitors. Journal of Applied Physics (2008); 104: 0949071-5.
  • K R Bhayani, S N Kale, Sumit Arora, Rajashree Rajagopal, H Mamgain, R Kaul-Ghanekar, Darshan C Kundaliya, S D Kulakarni, Renu Parischa, s D Dhole, S B Ogale and K M Paknikar. Protein and polymer immobilized La0.7Sr0.3MnO3 nanoparticles for possible biomedical applications. Nanotechnology (2007); 18:345101-7.
  • Jalota-Badhwar A, Kaul-Ghanekar R, Mogare D, Ramanmurthy, B, Paknikar K M and Chattopadhyay S. SMAR1 derived P44 peptide retains its tumor suppressor function through modulation of p53. J Biol Chem. 2007; 282 (13):9902-9913
  • Kaul-Ghanekar R,  Majumdar S, Jalota A, Gulati N, Dubey N, Saha B  and Chattopadhyay S. Abnormal V(D)J recombination of T cell receptor beta locus in SMAR1 transgenic mice. J Biol Chem. 2005; 280(10):9450-9.
  • Jalota A, Singh K, Pavithra L, Kaul-Ghanekar R, Jameel S and Chattopadhyay S. Tumor Suppressor SMAR1 Activates and Stabilizes p53 through Its Arginine-Serine-rich Motif. J Biol Chem. 2005; 280(16):16019-29.
  • Kaul-Ghanekar R, Jalota A, Pavithra L, Tucker P and Chattopadhyay S. SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCRbeta enhancer (Ebeta). Nucleic Acids Res. 2004; 32(16):4862-75.
  • Kaul R, Mukherjee S, Ahmed F, Bhat MK, Chhipa R, Galande S and Chattopadhyay S. Direct interaction with and activation of p53 by SMAR1 retards cell-cycle progression at G2/M phase and delays tumor growth in mice. Int J Cancer. 2003; 103(5): 606-15.(Cover page article)
  • Sarkar A, Chattopadhyay S, Kaul R, and Pal JK. Lead exposure and heat shock inhibit cell proliferation in human HeLa and K562 cells by inducing expression and activity of the heme-regulated eIF-2alpha kinase. J Biochem Mol Biol Biophys. 2002; 6(6): 391-6.
  • Chattopadhyay S, Kaul R, Charest A, Housman D and Chen J. SMAR1, a novel, alternatively spliced gene product, binds the Scaffold/Matrix-associated region at the T cell receptor beta locus. Genomics. 2000; 68(1): 93-6.

Chapter in book:

  • Ruchika Kaul-Ghanekar*, Snehal Suryavanshi, Prerna Raina. Early biomarkers in breast cancer in Cancer Biomarkers: Minimal and Non-Invasive Early Diagnosis and Prognosis. Taylor & Francis, 2014
  • Ruchika Kaul-Ghanekar*, Amit S Choudhari, Savita Pandita. Biomarkers for Early Detection of Cervical Cancer in Noninvasive Molecular Markers in Gynecologic Cancers. Taylor & Francis, 2015
  • Ruchika Kaul-Ghanekar*, Snehal Suryavanshi, Prerna Raina. Early biomarkers in breast cancer in Noninvasive Molecular Markers in Gynecologic Cancers. Taylor & Francis, 2015
  • Savita Pandita, Rashmi Deshpande, Shama Aphale, and Ruchika Kaul-Ghanekar. Role of Medicinal Plants in Targeting Important Signaling Pathways in Cervical Cancer in Holistic Healthcare Possibilities and Challenges, Apple Academic Press, USA, 2016.

General Articles:

  • Snehal A Suryavanshi and Ruchika Kaul-Ghanekar*. Current Scenario of Breast Cancer: An Overview. Bharati Vidyapeeth Deemed University Journal,2013, 10 (3): 16-29.
  • Amit S Choudhari and Ruchika Kaul-Ghanekar*. Cervical cancer Statistics and prevention Strategies: A special emphasis on Indian scenario. Bharati Vidyapeeth Deemed University Journal,2013, 10 (3): 96-103.
  • Kirtee Wani and Ruchika Kaul-Ghanekar*. Magnetic iron oxide nanoparticles: Role in cancer. Bharati Vidyapeeth Deemed University Journal,2013, 10 (2): 17-23.
  • Mansara P, Deshpande R, Kaul-Ghanekar R*, Ranjekar P. Role of Omega 3 fatty acids in the prevention of breast cancer: in vitro, in vivo and clinical studies. Bharati Vidyapeeth Deemed University Journal,2011, 9 (1): 10-18.
  • Koppikar S J, Harsulkar A, Kaul-Ghanekar R, Ranjekar P. “Exploring an anti-inflammatory potential of medicinal plants in osteoarthritis” Bharati Vidyapeeth Deemed University Journal, 2010,7(2):68-71.

*Corresponding author publications

Proceedings:

Cancer:Research Opportunities from Ayurveda and nutritional perspectives. 2008. Bharati Vidyapeeth Deemed University, Pune, Maharashtra.